Plasma glutathione peroxidase (GPx3) activity in the freshwater turtle Trachemys scripta elegans after isoflurane inhalation anesthesia

Introduction. Glutathione peroxidases are selenoenzymes which have a crucial role in the protection of animals against oxidative stress. Materials and Methods. From September 2017 to April 2018, group eight red-eared sliders were admitted at Clinic for Small Animals, Faculty Veterinary Medicine, University Belgrade elective diagnostic celioscopy. The turtles unknown age, weight from 1.20 kg 1.86 kg. anesthesia protocol involved using ketamine medetomidine, both low dosage (10 mg kg-1 0.1 kg-1, respectively) as induction, after was maintained isoflurane 3% (vapor setting) 100% oxygen (0.5 L min-1). Medetomidine reversed with atipamezole (0.2 kg-1), given intramuscularly. celioscopy performed according standard protocols. One day prior anesthesia, heparinized blood samples taken subcarapacial venous plexus venipuncture. second sampling took place three hours anesthetics administered. Results Conclusions. GPx3 activity plasmas (n=8) measured by coupled test described G?nzler et al. (1974). Data tested normality Shapiro-Wilk groups compared paired t-test. Blood plasma significantly higher (p=0.009) three-hour recovery period inhalation celioscopy, than before anesthesia. post-anesthesia activities were, on average, 80% measurements It can be concluded that statistically significant increase 91.02?36.05 mKat L-1 160.21?58.94 is due change saturation. This increased during procedure, thus exposing conditions high